目的 采用Box-Behnken响应面法优选牛至中百里香酚与香芹酚的提取工艺。方法 在单因素实验的基础上,以百里香酚与香芹酚提取率之和为评价指标,采用Box-Behnken设计法考察乙醇浓度、液固比和药材粒度对提取率的影响。结果 最佳的提取工艺参数为:乙醇浓度体积分数80%,液固比13∶1(mL/g),药材粒度为过40目筛,最高提取率为694.80 μg·g-1,与预测值的偏差较小。结论 所优选的提取方法操作简便、成本低、预测性良好,可为牛至中百里香酚与香芹酚的规模化大生产提供参考。
Abstract
OBJECTIVE To optimize the extraction process of thymol and carvacrol in Origanum vulgare by Box-Behnken design-response surface methodology. METHODS On the basis of single factor experiment, taking the sum of extraction rates of thymol and carvicol as the evaluation index, Box-Behnken design was used to investigate the effects of ethanol concentration, liquid-solid ratio and medicinal powder on the extraction rate. RESULTS The optimal extraction parameters were as follows: ethanol concentration was 80%, liquid to solid ratio was 13∶1 (mL/g), medicinal powder passing through 40 meshes was used, and the highest extraction rate was 694.80 μg·g-1, with a small deviation from the predicted value. CONCLUSION The optimal extraction method is simple, with low cost and good predictivity, and it can provide experimental basis for further large-scale production of thymol and carvacrol in Origanum vulgare.
关键词
牛至 /
牛至香酚 /
百里香酚 /
香芹酚 /
Box-Behnken响应面法 /
提取工艺
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Key words
Origanum vulgare /
oregano phenol /
thymol /
carvacrol /
Box-Behnken design-response surface methodology /
extraction process
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中图分类号：
R284.2
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参考文献
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脚注
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基金
福建省科技厅科技平台建设项目资助(2015Y2001-57);青海省重点研发与转化计划项目资助(2020-SF-C34);福建中医药大学校管课题-科研创新平台专项资助(X2019013)
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